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KMID : 0363020080380030543
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Journal of Korean Academy of Periodontology
2008 Volume.38 No. 3 p.543 ~ p.550
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Pro-inflammatory cytokine expression in human gingival fibroblasts by Tannerella forsythia whole bacteria, membrane proteins, and Lipopolysaccharide
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Kim Jung-Eun
Lee Sung-Hoon
Choi Bong-Kyu
Koo Ki-Tae
Kim Tae-Il
Lee Yong-Moo
Ku Young
Chung Chong-Pyoung
Rhyu In-Chul
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Abstract
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Purpose: The purpose of this study was to investigate induction of cytokine expression in human gingival fibroblasts (HGFs) by whole cell and the components of T. forsythia.
Material and Methods: After HGFs were treated with lipopolysaccharide (LPS), membrane protein isolated from T. forsythia or culture media of T. forsythia, the induction of interleukin (IL)-1, IL-6 and IL-8 was examined with real-time PCR and ELISA. Their induction ability of cytokines was compared with whole bacteria.
Result: The expression of IL-6 and IL-8 was significantly induced in HGFs by whole bacteria and membrane protein. The expression of IL-1β was induced by membrane protein of T. forsythia, not by whole bacteria. LPS and condition media of T. forsythia slightly activated HGFs.
Conclusion: The membrane protein of T. forsythia could be one of virulence factors.
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KEYWORD
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Tannerella forsythia, virulence factors, human gingival fibroblasts
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